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Our laboratory demonstrated that SERT and αvβ3 receptors colocalize in midbrain presynaptic terminals (Mazalouskas et al., 2015), suggesting that SERT is localized to multiprotein complexes coordinated by integrin αvβthree in midbrain synapses. Preceding scientific studies recommend that in platelets, the Affiliation of SERT with activated αIIbβthree integrins, and its localization to adhesion complexes, are correlated with greater uptake action (Carneiro et al.
Sizeable genotype discrepancies in the amount of synapses are indicated in italics. Structured illumination microscopy of WT and KI midbrain slices was used to quantify the colocalization on the listed proteins within just SERT-good synapses.
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The context-dependent nature of SERT regulation is underscored from the lack of colocalization of SERT with focal adhesion proteins in KI synapses. This means which the association of SERT with integrin signaling complexes just isn't essential for the regulation on the transporter by constitutive signaling downstream with the “primed” Pro32Pro33 mutant.
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